EC4 EQUAL PROJECT - Training Course Bari (Italy)

Preliminary Announcement

EQUAL Training Course Bari (Italy)

A three days meeting which includes practical activities in the laboratory (revision to the EQUAL-quant Programme) and lectures on: Biomarkers in Oncology: Detection and Clinical Meaning

Venue	Scientific sessions Library National Cancer Institute Via Amendola 209 70126 Bari Practical activities Clinical Experimental Oncology Laboratory National Cancer Institute via Amendola 209, 70126 Bari
Date	September 27-29, 2005
Coordinators	Scientific Coordinator Dr. Angelo Paradiso, Clinical Experimental Oncology Laboratory, National Cancer Institute-Bari Coordinator of Practical Activities Dr. Stefania Tommasi Clinical Experimental Oncology Laboratory, National Cancer Institute-Bari
General Information of the Training Course	The programme will include lectures on clinical molecular diagnostics by Real Time PCR, as well as practical activities in laboratory related to the EQUAL-quant programme. Practical activities will be reserved to participants in the EQUAL-quant programme.
Scientific topics	The introduction of Real Time PCR as an innovative assay to detect cell hosts and to quantify genes has been assuming a great importance in the clinical routine of many laboratories. Example of clinical application in several fields are now available. In particular, Real Time PCR is used in oncology to detect prognostic factors in terms of gene expression, drug targets directly related to gene amplification and single nucleotide mutation, etc. Furthermore, several application for the determination of minimal/micrometastatic disease in several tissue compartments will be discussed. The formal sessions of the course will address the following topics: Interpretation of Real Time results and their technical troubles Methodological approaches for the quantitation of human DNA sequence of multigenes analysis Potential advantages and limits of their application in the clinical setting: in oncology, surgical oncology, microbiology Relevance of tissue banking
Practical activities	For EQUAL participants only The main aim of the EQUAL Training Course in Bari is the revision of the actions of the EQUAL-quant Programme and the discussion of the results of this survey. 8 hours of the Bari EQUAL-quant Training Course will be dedicated to practical workshop. The methodological skills required to improve analytical performance of the EQUAL-quant Programme will be reviewed during the Course and then tested allowing the participants to perform for the second time the EQUAL-quant Programme. Comparison of the results after training will be used to judge the efficacy of the Course. A specifically defined questionnaire will be also distributed to the participants at the end of the Course.
Scientific Programme
27 September 2005
	9.00	Welcome
	9.30-10.30	Mario Pazzagli, Florence: Overview of Equal Project: Seq, Qual, Quant
	10.30-11.30	Simon Ramsden, Manchester: Equal-Quant results
		Coffee Break
	Introduction to practical activities
	11.45-12.45	Paolo Verderio, Milano; Sarah Daly, Manchester: Interpretation of Quant results and forms
	12.45-13.45	Claudio Orlando, Florence; Stefania Tommasi, Bari: Technical troubles discussion
		Lunch
	Practical workshop
	15.00-16.00	Nicola Cirenei, Monza: Data format and interpretation
	16.00-17.00	Angelo De Biasi, Roma: Chemical and technical peculiarities
	17.00-18.00	Roberta Madej, Pleasanton: Use of external RNA controls in gene expression assay: proposed guideline
	Closing remarks
28 September 2005
	9.30-10-30	Vladmir Palicka, Hradec Králové: Quantitative PCR in microbiology
	10.30-11.30	Claudio Orlando, Florence: Quantitative PCR in oncology
		Coffee Break
	Towards of global QC
	11.45-12.45	Pierre Martin, Marseille: The problem of internal standard
	12.45-13.45	Mario Plebani, Padua: Pre-analytical troubles and PCR assay
		Lunch
	Practical workshop
	15.00-16.00	Nicola Cirenei, Monza: Gene expression in Real Time: evaluation and analysis
	16.00-17.00	Serenella Eppenberger, Basel: Multigenes analysis quantitative Real Time PCR
	17.00-18.00	Philippe Planchon, Maurens Scopont: Intrasurgical application of Rela Time PCR
	Closing remarks
29 September 2005
	9.00-10.00	Angelo Paradiso, Bari: Relevance of tissue banking I 3rd millennium activity
	10.00-11.00	Maria Grazia Daidone, Milano: International tumor tissue bank
	11.00-12.00	Giuseppe Pelagio, Bari: Handling procedures of tissues for PCR approaches
Questionnaire Closing Remarks
Registration Form (no registration fee)	Do you want to register as an EQUAL- quant participant? click here Do you want to register as a non-EQUAL participant? click here
Practical information	about travel and accommodation will be available from January 2005
Contact us	Dr. Angelo Paradiso Clinical Experimental Oncology Laboratory National Cancer Institute v. Amendola 209 70126 Bari, Italy e-mail: a.paradiso@oncologico.bari.it Dr. Stefania Tommasi Clinical Experimental Oncology Laboratory National Cancer Institute v. Amendola 209 70126 Bari, Italy e-mail: equal.nci.bari@virgilio.it

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